Pan-Caspase Inhibitor. Z-VAD-FMK acts as an effective irreversible caspase inhibitor with no cytotoxic effects and, therefore, is a useful tool for studying caspase activity.
|Alternative Name:||Caspase family inhibitor (fluoromethylketone), Z-Val-Ala-DL-Asp-FMK|
|Application Notes:||Can be used as a broad spectrum caspase inhibitor in conjunction with apoptosis detection assays.|
|Solubility:||Soluble in DMSO or acetonitrile (10mg/ml).|
|Long Term Storage:||-20°C|
|Use/Stability:||Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution.|
|Handling:||After reconstitution, prepare aliquots and store at -20°C.|
Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2µl of this stock solution to 1ml of culture medium containing cells to give 20µM final concentration. Effective final concentrations are estimated to be 5-20µM. If a particular caspase inhibitor is applied at higher concentrations specificity for an individual caspase or even for the caspase family (> 100µM) might be compromised. DMSO concentration above 0.2% may cause cellular toxicity thus masking the effect of the caspase inhibitor. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease.