ENZO ▼Y-27632 . dihydrochloride
Y-27632 is a highly potent, cell permeable, ive and ATP competitive inhibitor of ROCK1 and ROCK2 (IC50=800nM). It acts as a potent inhibitor of agonist-induced Ca2+sensitization of myosin phosphorylation and smooth muscle contractions, blocking cell spreading and suppresses RhoA-induced formation of stress fibers in hepatic stellate cells. Y-27632 significantly reduces the increase of inflammatory cytokines after reperfusion, preventing the development of acute renal failure. There are numerous application for this compound including: cardioprotective effects, suppression of tumor cell invasion, inhibition of superoxide production, mimicking effect of β-agonists on human cells, and an antinociceptive effect. There has been a growing interest in Y-27632 for use in stem cell self-renewal and reprogramming. It is known to increase the survival rate of human embryonic stem cells undergoing cryopreservation.
(R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide . 2HCl
|Formula:||C14H21N3O . 2HCl|
|MW:||247.3 . 73.0|
|Purity:||≥98% (HPLC (UV))|
|Purity Detail:||Enantiomeric excess ≥96%|
|Identity:||Determined by MS, 1H- and 13C-NMR.|
|Appearance:||White to pale yellow powder.|
|Solubility:||Soluble in DMSO (25mg/ml), methanol (25mg/ml), acetonitrile (<1mg/ml), dioxan (<1mg/ml), water (very soluble).|
|Long Term Storage:||-20°C|
|Use/Stability:||Stock solutions are stable for up to 1 month when stored at -20°C.|
Protect from light. Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C.