MG-132 is a potent, cell permeable and ive proteasome inhibitor (Ki = 4nM).1 It inhibits NF-κB activation by preventing IκB degradation (IC50 = 3μM). The peptide blocks degradation of short-lived proteins, which in turn induces HSP and ER chaperone expression, leading to thermotolerance (1μM MG-132, 2 h.). It also stimulates neurite outgrowth in PC12 cells (20nM optimal). The peptide has also been reported to increase the survival rate of mesenchymal stem cells following their transplantation. IC50’s for inhibition of Suc-LLVY-AMC and Z-LLL-AMC cleaving activities of proteasome were 0.85 and 0.1μM respectively. The ubiquitin-proteasome system (UPS) and autophagy serve as two complementary, reciprocally regulated protein degradation systems, thus blockade of UPS by MG-132 activates autophagy.
|Solubility:||Soluble in DMSO (25mg/ml) or 100% ethanol (25mg/ml).|
|Shipping:||Shipped on Dry Ice|
|Long Term Storage:||-80°C|
Solutions are stable for up to one week if stored at -20°C. Solutions are stable for up to two months if stored at -80°C.