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ENZO ▼ROS-ID® Total ROS detection kit


ENZO ▼ROS-ID® Total ROS detection kit	브랜드	ENZO
	품명	ROS-ID® Total ROS detection kit
	CAT. NO	ENZ-51011	분류	detection kit
	규격	1Kit	재고	-
	소비자가(₩)		CAS. NO
	온도	-20℃	위험물
	수입발주일	매주 목요일 오후 5시
	주의사항	Short Term Storage:-20°C, Long Term Storage:-80°C

Information
Enzo Life Sciences’ ROS-ID^® Total ROS detection kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry. The kit includes Oxidative Stress Detection Reagent (Green) as the major component. This non-fluorescent, cell-permeable total ROS detection dye reacts directly with a wide range of reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals, yielding a green fluorescent product indicative of cellular production of different ROS/RNS types. The kit is not designed to detect superoxide, reactive chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm), or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. The Total ROS Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in solution).

Alternative Name: Reactive oxygen species

Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

Application Notes: This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

Quality Control: A sample from each lot of ROS-ID^® Total ROS detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm).
The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright green fluorescence in the cytoplasm. Cells pretreated with the ROS inhibitor don’t demonstrate any green fluorescence signal upon induction.

Quantity: 200 fluorescence microscopy assays or 50 flow cytometry assays.

Use/Stability: With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Handling: Protect from light. Avoid freeze/thaw cycles.

Shipping: Shipped on Dry Ice

Short Term Storage: -20°C

Long Term Storage: -80°C

Kit/Set Contains: Oxidative Stress Detection Reagent (Green), 300 nmoles
ROS Inducer (Pyocyanin), 1 µmole
ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg
Wash Buffer Salts, 1 pack

Technical Info/Product Notes:
The ROS-ID^® Total ROS detection kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^®reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Manual / MSDS

물품 이상, 파손의 경우 제품 수령 후 24시간 내에 연락 주시기 바랍니다.
교환과 반품은 물품 수령 후 2주일 이내에 가능합니다. 단, 수입발주 품목은 교환/반품이 불가합니다.
자세한 사항에 대해서는 동인바이오텍 대표번호 (02-431-7375)로 연락 주시기 바랍니다.

Alternative Name:	Reactive oxygen species

Applications:	Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

Application Notes:	This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

Quality Control:	A sample from each lot of ROS-ID^® Total ROS detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm). The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright green fluorescence in the cytoplasm. Cells pretreated with the ROS inhibitor don’t demonstrate any green fluorescence signal upon induction.

Quantity:	200 fluorescence microscopy assays or 50 flow cytometry assays.

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Handling:	Protect from light. Avoid freeze/thaw cycles.

Shipping:	Shipped on Dry Ice

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Kit/Set Contains:	Oxidative Stress Detection Reagent (Green), 300 nmoles ROS Inducer (Pyocyanin), 1 µmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack

Technical Info/Product Notes:	The ROS-ID^® Total ROS detection kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^®reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. Manual / MSDS

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