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ENZO ▼CYTO-ID® Autophagy detection kit

ENZO ▼CYTO-ID® Autophagy detection kit

브랜드 ENZO
품명 CYTO-ID® Autophagy detection kit
CAT. NO ENZ-51031-K200 분류 assay kit
규격 200tests 재고 -
소비자가(₩) CAS. NO
온도 -20℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항 Short Term Storage:-20°C, Long Term Storage:-80°C

Information

CYTO-ID® Autophagy Detection Kit measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that ively labels accumulated autophagic vacuoles. The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes). The assay offers a rapid and quantitative approach to monitoring autophagy in live cells without the need for cell transfection.


Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
 
Application Notes:The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.
 
Quality Control:A sample from each lot of CYTO-ID® Autophagy detection kit is used to stain Jurkat cells and analyzed by flow cytometry, using the procedures described in the user manual. The MAF values for the samples were greater than 25. The percentage of viable cells in the control samples is > 90% and > 80% in the treated samples.
 
Quantity:For -K200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.

For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays.
 
Use/Stability:With proper storage, the kit components are stable for one year from date of receipt.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Kit/Set Contains:CYTO-ID® Green Detection Reagent
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer
 
Scientific Background:

Autophagy is a stress-induced protective mechanism. Less active under basal conditions, the mechanism is utilized by eukaryotic cells through lysosome-mediated bulk degradation of cellular contents when subjected to certain hostile conditions such as nutrient depletion and chemical or environmental stress. The role of increased autophagic activity in the pathology of cancer, neurodegeneration, cardiovascular disease and diabetes has become widely recognized and commonly studied. Induction of autophagic flux can be visualized by enhanced accumulation of autophagic vesicles if lysosomal function is inhibited, preventing removal of these vesicles.

 

 

 

Manual  / MSDS 

 

 

 

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