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LSbio ▼Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2486

LSbio ▼Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2486

브랜드 LSbio
품명 Anti-MMP9 / Gelatinase B Antibody IHC-plus™ LS-B2486
CAT. NO LS-B2486-50 분류 Antibody
규격 50 μg 재고 -
소비자가(₩) 945,000 CAS. NO
온도 4℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항 +4°C or -20°C, Avoid repeated freezing and thawing.

 

Rat MMP9 / Gelatinase B
Rabbit
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with APAPCBiotinFITCHRPPE.
Protein A purified
Unmodified

 

    • IHC - Paraffin (5 µg/ml)
    • IHC - Frozen (1:50)
    • Western blot (1:1000)
    • Immunoprecipitation (1:250)
    • (applications tested for the base form of this product only)

E. coli expressed active rat 92kD type IV collagenase (catalytic domain).
Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.
Immunohistochemistry: LS-B2486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2486 was determined to be 5 ug/ml. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

PBS, 0.05% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

 



Data sheet 


 

 

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