동인바이오텍 쇼핑몰

BPS ▼HDAC1 Fluorogenic Assay Kit	브랜드	BPS
품명	HDAC1 Fluorogenic Assay Kit
CAT. NO	50061	분류	assay kit
규격	96 reactions	재고	-
소비자가(₩)		CAS. NO
온도	-80℃ -20℃ R.T.	위험물
수입발주일	목요일 오후 5시
주의사항

Background:

HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.

Information:

The Fluorogenic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC1. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

UniProt

Q13547

Synonym(s):

histone deacteylase, HDAC1

Contraindications:

DMSO >1%, strong acids or bases, ionic detergents, high salt

Format:

COMPONENTS:

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC1 for drug discovery and HTS applications.

Reference(s):

1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.
2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar;6(3): 238-243.

Product Specific Reference(s):
1. Hsieh, IN., et al., Cell Death Dis. 2014 Apr 10;5.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Deacetylase

Product Type:

Protocol

Assay Kit

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