LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of tranional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
The LSD1 Assay Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. The key to the LSD1 Assay Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (λex=530±13 nm, λem=590±18 nm) is measured using a plate reader.
KDM1A, AOF2, LSD1, BHC110, LSD
The LSD1 Assay Kit is provided in a convenient 384-well format, with purified LSD1, AmplexRed reagent, peroxidase, and LSD1 assay buffer for 384 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
1. Forneris F, et al. J Biol Chem 2006; 281(46):35289-95. 2. Zhou M, et al. Anal Biochem 1997; 253(2):162-8.