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BPS ▼Human IDO1 Inhibitor Screening Assay Kit

BPS ▼Human IDO1 Inhibitor Screening Assay Kit

브랜드 BPS
품명 Human IDO1 Inhibitor Screening Assay Kit
CAT. NO 72021 분류 assay kit
규격 96 Reactions 재고 -
소비자가(₩) 1,450,000 CAS. NO
온도 -80℃ R.T. 위험물
수입발주일 목요일 오후 5시
주의사항
Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Information:

The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=321 nm.

Synonym(s):
Indoleamine 2,3-dioxygenase 1
Supplied As:
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
Contraindications:
DMSO >0.5%, strong acids or bases, ionic detergents, high salt
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Useful for the study of IDO1 enzymology, screening inhibitors, and ivity profiling.
Reference(s):

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J Biomol Screen. 2014; 19(9): 1266-74.

Application Reference(s):
1. Synthesis and Molecular Modeling Studies of N-Hydroxyindazolecarboximidamides as Novel Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors (2017)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:




Protocol


Assay Kit

 

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