Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.
The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
tryptophan 5-hyrdoxylase, TRPH
Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266. 2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.
MATERIALS REQUIRED BUT NOT SUPPLIED: Microplate reader capable of measuring Fluorescence