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BPS ▼ACC2, His-tag


BPS ▼ACC2, His-tag	브랜드	BPS
	품명	ACC2, His-tag
	CAT. NO	50201	분류	Recombinant Protein
	규격	10 μg	재고	-
	소비자가(₩)		CAS. NO
	온도	-80℃	위험물
	수입발주일	목요일 오후 5시
	주의사항

Information:

Human acetyl-Coenzyme A carboxylase alpha tran variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Synonym(s):

acetyl-coa carboxylase, acetyl coa, acetyl coenzyme a, ACC2, acetyl-Coenzyme A carboxylase alpha tran variant 2, ACC-2, ACC 2, ACC -2

Specific Activity:

≥20 pmoles/min/µg

Assay Conditions:

Assay was done using ADP-Glo (Promega) reagents with 20 μM ATP, 20 μM acetyl-CoA and 12 mM NaHCO3 in 25 μL ACC assay buffer (30 mM HEPES pH=7.4, 2 mM MgCl2, 2 mM DTT, 0.01% Brij35 and 2 mM Sodium citrate). Reaction at room temperature for 30 min.

Formulation:

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 100 mM Imidazole.

Format:

Aqueous buffer solution

Storage / Stability:

>6 months at –80°C

Application(s):

Useful for the study of enzyme kinetics, screening inhibitors, and ivity profiling.

Reference(s):

1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates(2015)

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Metabolic Enzymes-Carboxylase

Product Type:

Data sheet

Recombinant Protein

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