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BPS ▼ACC2, His-tag

BPS ▼ACC2, His-tag

브랜드 BPS
품명 ACC2, His-tag
CAT. NO 50201 분류 Recombinant Protein
규격 10 μg 재고 -
소비자가(₩) 950,000 CAS. NO
온도 -80℃ 위험물
수입발주일 목요일 오후 5시
주의사항
Information:
Human acetyl-Coenzyme A carboxylase alpha tran variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
acetyl-coa carboxylase, acetyl coa, acetyl coenzyme a, ACC2, acetyl-Coenzyme A carboxylase alpha tran variant 2, ACC-2, ACC 2, ACC -2
Specific Activity:
≥20 pmoles/min/µg
Assay Conditions:
Assay was done using ADP-Glo (Promega) reagents with 20 μM ATP, 20 μM acetyl-CoA and 12 mM NaHCO3 in 25 μL ACC assay buffer (30 mM HEPES pH=7.4, 2 mM MgCl2, 2 mM DTT, 0.01% Brij35 and 2 mM Sodium citrate). Reaction at room temperature for 30 min.
Formulation:
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 100 mM Imidazole.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and ivity profiling.
Reference(s):

1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).

Application Reference(s): 
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates(2015)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Carboxylase
Product Type:




Data sheet


Recombinant Protein

 

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