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LSbio ▼Human F8 / FVIII / Factor VIII ELISA Kit (Sandwich ELISA) - LS-F10415

LSbio ▼Human F8 / FVIII / Factor VIII ELISA Kit (Sandwich ELISA) - LS-F10415

브랜드 LSbio
품명 Human F8 / FVIII / Factor VIII ELISA Kit (Sandwich ELISA) - LS-F10415
CAT. NO LS-F10415-1 분류
규격 1 plate 재고 -
소비자가(₩) 1,260,000 CAS. NO
온도 위험물
수입발주일 매주 목요일 오후 5시
주의사항
Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
F8 / FVIII / Factor VIII
Reactivity
 
Human
Manual
 
Intended Sample Types
 
Plasma
Sample Dilution
 
Samples giving human Factor VIII levels above 0.84 IU/ml should be diluted in blocking buffer before use. 1:8 and 1:16 dilutions for normal plasma, or
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.0015 - 0.84 IU/ml
Sensitivity
 
0.00086 IU/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 
  • Coated 96-well Strip Plate
  • 10X Wash buffer
  • Human Factor VIII Standard (Lyophilized)
  • Anti-human Factor VIII Primary Antibody (Lyophilized)
  • HRP-conjugated Streptavidin
  • TMB Substrate
  • Adhesive Plate Sealers
Background
 
Factor VIII (aka Factor VIII:C or Antihemophilic Globulin) is a glycoprotein zymogen that circulates in a stabilized non-covalent complex with von Willebrand Factor (vWF). Following activation by thrombin or Factor Xa, Factor VIIIa dissociates from vWF and catalyzes the activation of Factor X by Factor IXa in the amplification phase of coagulation. Factor VIIIa activity is quickly decreased by spontaneous dissociation and proteolytic degradation by activated Protein C, Factor Xa and Factor IXa. Hemophilia A is caused by mutations in the Factor VIII gene; a majority of patients have decreased Factor VIII plasma levels while 5% of patients have normal levels of nonfunctioning protein.
Restrictions
 
For research use only.

 

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