ENZO ▼PGE2 ELISA kit
The PGE2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Screen inhibitors of COX II activity by measuring the levels of downstream PGE2. Commercially available since 1992, this kit is widely cited in peer-reviewed publications. The non-radioactive ready-to-use liquid color-coded reagents reduce errors.
|Alternative Name:||Prostaglandin E2|
|Sensitivity:||13.4 pg/ml (range 39.1 - 2,500 pg/ml)|
|Assay Time:||<3 hours|
|Applications:||ELISA, Colorimetric detection|
|Application Notes:||For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue.|
|Species reactivity:||Species independent|
|Use/Stability:||Store all components at +4°C, except standard and conjugate at -20°C.|
|Shipping:||Shipped on Blue Ice|
|Contents:||GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution|
Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.