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ENZO ▼Protein A ELISA kit

ENZO ▼Protein A ELISA kit

브랜드 ENZO
품명 Protein A ELISA kit
CAT. NO ADI-900-057 분류 ELISA kit
규격 1 x 96wells 재고 -
소비자가(₩) CAS. NO
온도 4℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항

Information

The Protein A EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Ultra-sensitive quantification ( < 1 ppm ) of Protein A residuals in purified humanized monoclonal antibody preparations. This kit recognizes four different Protein A constructs. 

 

 

 

Sensitivity:9.01 pg/ml (range 15.63 - 1,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate,Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.
 
Technical Info/Product Notes:

Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided.

 

 

 

 

Manual  / MSDS 


  

 

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