ENZO ▼PKA kinase activity kit
|Alternative Name:||Protein kinase A, cAMP-dependent protein kinase|
|Application Notes:||For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species.|
|Species reactivity:||Species independent|
|Use/Stability:||Store all components at +4°, except active kinase at -80°.|
|Shipping:||Shipped on Dry Ice|
|Contents:||Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2|
PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.