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ENZO ▼cAMP complete ELISA kit

ENZO ▼cAMP complete ELISA kit

브랜드 ENZO
품명 cAMP complete ELISA kit
CAT. NO ADI-900-163 분류 ELISA kit
규격 1 x 96wells 재고 -
소비자가(₩) 629,000 CAS. NO
온도 -20℃ 4℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항 Store all components at +4°C, except standard and conjugate at -20°C

 

Information

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.


Alternative Name:cyclic AMP
 
Sensitivity:Assay Buffer : 0.30 pmol/ml (non-acetylated) 0.039 pmol/ml (acetylated) HCl: 0.39 pmol/ml (non-acetylated) 0.037 pmol/ml (acetylated) (range 0.78 - 200 pmol/ml (non-acetylated); 0.078 - 20 pmol/ml (acetylated))
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of cAMP in cell lysates, culture supernatants, serum, saliva, and tissue from any species.
 
Species reactivity:Species independent
 
Crossreactivity:cyclic AMP (100%), AMP (0.33%), ATP (0.12%) and <0.001%: cyclic GMP, GMP, GTP, cyclic UMP, CTP
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Contents:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Assay buffer 2, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Triethylamine, Acetic anhydride
 
Scientific Background:Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.




Manual  / MSDS  


 

 

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