Enzyme linked immunosorbent assay (ELISA) for the detection of Mouse IgG in serum or plasma. Other biological fluids that contain Mouse IgG, such as urine, feces, saliva, may be suitable samples. Due to idiotypic and allotypic variations, this product may not accurately quantitate hybridomas, monoclonals or myelomas. The Set contains sufficient components to perform 1000 single well assays. The set performance has been optimized for the stated protocol using the materials listed and standard dilutions from 500 – 7.8 ng/ml of Mouse IgG. For alternative assay conditions, the operator must determine appropriate dilutions of reagents.
• Affinity purified Goat anti-Mouse IgG-Fc Coating Antibody, 1 ml at 1 mg/ml (1:100)
• Mouse Reference Serum, 0.1 ml
• HRP Conjugated Goat anti-Mouse IgG-Fc Detection Antibody, 0.1 ml at 1 mg/ml (1:100,000)