Enzyme linked immunosorbent assay (ELISA) for the detection of Human IgG in serum, plasma, or milk. Other biological fluids that contain Human IgG, such as urine, feces, saliva, may be suitable samples. The Set contains sufficient components to perform 1000 single well assays. The set performance has been optimized for the stated protocol using the materials listed and standard dilutions from 500 – 7.8 ng/ml of Human IgG. For alternative assay conditions, the operator must determine appropriate dilutions of reagents.
• Affinity purified Goat anti-Human IgG-Fc Coating Antibody, 1 ml at 1 mg/ml (1:100)
• Human Reference Serum, 0.1 ml
• HRP Conjugated Goat anti-Human IgG-Fc Detection Antibody, 0.1 ml at 1 mg/ml (1:150,000)