Viromer® BLUE was developed for the siRNA transfection and is optimized for binding the size of small oligonucleotides like miRNA and siRNA. It is based on a medium sized branched polymer and the neutral charge of Viromer BLUE : miRNA and siRNA transfection complexes results in no aggregation and high performance in suspension cells like primary microglia cells. Viromer® BLUE achieves the best miRNA or siRNA transfection results in classical cell lines widely used in cell biology such as HEK-293, HeLa and CHO as well as in challenging primary cells like mesenchymal stem cells, macrophages, monocytes, myoblasts and hepatocytes. By using Viromer® BLUE a highly efficient miRNA and siRNA transfection is possible without the use of enhancers. Based on an active endosome escape mechanism siRNA transfection becomes more efficient compared to standard reagents as more of introduced siRNA/miRNA molecules reach the cytoplasma. Due to lowest toxicity and superior efficiency, Viromer® BLUE turns a hardly-possible miRNA and siRNA transfection into a superior one.

EXCELLENT RESULTS IN siRNA TRANSFECTION OF HARD-TO-TRANSFECT CELLS

Proprietary Viromer® BLUE miRNA and siRNA transfection technology has already convinced numerous research groups by providing excellent results on cell lines and primary cells that were thought to be impossible to transfect. siRNA transfection of human primary macrophages and human primary mesenchymal stem cells results in 70% knock down efficiency using Viromer® BLUE.

For primary skeletal myoblasts, primary hepatocytes, RAW264.7 mouse macrophages, melanoma cells, Hs74T gastric carcinoma cells as well as glioblastoma cell lines NCH82 and NCH149 a siRNA transfection efficiency >90% can be achieved using Viromer® BLUE.

 APPLICATIONS

• RNA interference with siRNA
• regulation of protein expression via miRNA
• cancer research
• stem cell research
• cell signaling