동인바이오텍 쇼핑몰

BPS ▼Human TDO Inhibitor Screening Assay Kit	브랜드	BPS
품명	Human TDO Inhibitor Screening Assay Kit
CAT. NO	72023	분류	assay kit
규격	96 reactions	재고	-
소비자가(₩)		CAS. NO
온도	-80℃ R.T.	위험물
수입발주일	목요일 오후 5시
주의사항

Background:

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

Information:

The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation activity is measured by measuring the absorption of reaction product at λ = 320-325 nm.

Synonym(s):

TDO2, tryptophan 2,3 dioxygenase

Format:

COMPONENTS:

Instructions for use:

See product datasheet for detailed protocol.

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Useful for the study of TDO enzymology, screening inhibitors and ivity profiling.

Reference(s):

Li, J.S., et al. Arch Insect Biochem Physiol. 2007 Feb; 64(2): 74–87

Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Spectrophotometer capable of measuring absorbance at = 320-325 nm.

Warning(s):

DMSO >0.5%, strong acids or bases, ionic detergents, high salt
Avoid freeze/thaw cycles.

Scientific Category:

Immunotherapy, Metabolic enzyme

Product Type:

Protocol

Assay Kit

BRAND