Important Notes:

1. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer.
2. Add the required volume of ethanol (96~100%) to Wash Buffer before use.
3. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.

Gel Extraction Protocol: For extraction of DNA fragments from agarose gel
Please Read Important Notes Before Starting Following Steps.

HINT: Prepare a 55 °C dry bath or water bath for step 4.
1. Excise the agarose gel with a clean scalpel.
• Remove the extra agarose gel to minimize the size of the gel slice.
2. Transfer up to 300 mg of the gel slice into a microcentrifuge tube.(not provided).
• The maximum volume of the gel slice is 300mg.
3. Add 500 μl of FADF Buffer to the sample and mix by vortexing.
• For > 2% agarose gels, add 1000 μl of FADF Buffer.
4. Incubate at 55 °C for 5 ~10 minutes and vortex the tube every 2 ~ 3 minutes until the gel slice dissolved completely.
• During incubation, interval vortexing can accelerate the gel dissolved.
• Make sure that the gel slice has been dissolved completely before proceed the next step.
• After gel dissolved, make sure that the color of sample mixture is yellow. If the color is violet, add 10 μl of sodium acetate, 3M,
pH 5.0. Mix well to make the color of sample mixture turned to yellow.
5. Cool down the sample mixture to room temperature. And place a FADF Column into a Collection Tube.
6. Transfer 800 μl of the sample mixture to the FADF Column. Centrifuge at 11,000 x g for 30 seconds, then discard the
flow-through.
• If the sample mixture is more than 800 μl, repeat this step for the rest of the sample mixture.
7. Add 750 μl of Wash Buffer (ethanol added) to the FADF Column. Centrifuge at 11,000 x g for 30 seconds, then discard the
flow-through.
• Make sure that ethanol (96-100 %) has been added into Wash Buffer when first use.
8. Centrifuge again at full speed (~ 18,000 x g) for an additional 3 minutes to dry the column matrix.
• Important step ! The residual liquid should be removed thoroughly on this step.
9. Place the FADF Column to a new microcentrifuge tube (not provided).
10. Add 40 μl of Elution Buffer or ddH2O to the membrane center of the FADF Column. Stand the FADF Column for 1 min.
• Important step ! For effective elution, make sure that the elution solution is dispensed onto the membrane center and is
absorbed completely.
• Important : Do not elute the DNA using less than suggested volume (40 μl). It will lower the final yield.

PCR Clean-Up Protocol: For purification of PCR products or reaction mixtures
Please Read Important Notes Before Starting Following Steps

1. Transfer up to 100 μl of PCR product (excluding oil) to a microcentrifuge tube (not provided) and add 5 volumes of
FADF Buffer, mix well by vortexing.
• For example, Add 250 μl of FADF Buffer to 50 μl of PCR product.
• The maximum volume of PCR product is 100 μl (excluding oil). Do not excess this limit. If PCR product is more than 100 μl,
separate it into multiple tubes.
2. Place a FADF column into a Collection Tube.
3. Transfer the sample mixture to the FADF Column. Centrifuge at 11,000 x g for 30 seconds, then discard the flow-through.
4. Add 750 μl of Wash Buffer (ethanol added) to the FADF Column. Centrifuge at 11,000 x g for 30 seconds, then discard the
flow-through.
• Make sure that ethanol (96-100 %) has been added into Wash Buffer when first open.
5. Centrifuge again at full speed (~18,000 x g) for an additional 3 minutes to dry the column matrix.
• Important step ! The residual liquid should be removed thoroughly on this step.
6. Place the FADF Column to a new microcentrifuge tube (not provided).
7. Add 40 μl of Elution Buffer or ddH2O to the membrane center of the FADF Column. Stand the FADF Column for 1 min.
• Important step ! For effective elution, make sure that the elution solution is dispensed onto the membrane center and is
absorbed completely.
• Important : Do not elute the DNA using less than suggested volume (40 μl). It will lower the final yield.
8. Centrifuge at full speed (~18,000 x g) for 1 min to elute the DNA.
11. Centrifuge at full speed (~ 18,000 x g) for 1 min to elute the DNA. 

MSDS / Datasheet