Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Immunogen
LMX1B antibody was raised against synthetic peptide QQALTTSCYFRDRK from an internal region of human LMX1B (NP_002307.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Opossum, Chicken (100%); Xenopus (93%); Stickleback, Zebrafish (86%).
Blocking Peptide
LS-E26838 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6479. Useful for pre-absorption and neutralization of the antibodys antigen binding site.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 100kDa band in Mouse Kidney lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6kDa according to Human NP_002307.1 and to Mouse NP_034855.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.