smobio ▼ExcelRT™ Reverse Tranion Kit |
브랜드 | smobio | ||
---|---|---|---|---|
품명 | ExcelRT™ Reverse Tranion Kit | |||
CAT. NO | RP1300 | 분류 | ||
규격 | 100 RXN | 재고 | - | |
소비자가(₩) | CAS. NO | |||
온도 | 위험물 | |||
수입발주일 | 수시 발주 | |||
주의사항 |
Information
ExcelRT™ Reverse Tranion Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Tranase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Tranase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse tranase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
Features
• Contains all components for reverse tranion
• High yield
• Thermostable, up to 50°C, during first strand synthesis
• High processivity, generating cDNA up to 8 kb
• Reduced RNase H ribonuclease activity
Contents
Component
Volume
Reverse Tranase (200 U/μl) | 100 µl |
RNase Inhibitor (20 U/μl) | 100 µl |
5X RT Buffer (DTT) | 500 µl |
dNTPs (10 mM each) | 200 µl |
Oligo (dT) 20 (50 μM) | 100 µl |
Random Hexamers (100 μM) | 100 µl |
DEPC-Treated H2O | 2X1 ml |
Storage
-20°C for 24 months
Storage Buffer
Reverse Tranase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol