BRAND

smobio ▼ExcelRT™ Reverse Tranion Kit

브랜드 smobio
품명 ExcelRT™ Reverse Tranion Kit
CAT. NO RP1300 분류
규격 100 RXN 재고 -
소비자가(₩) CAS. NO
온도 위험물
수입발주일 수시 발주
주의사항
  • RP1300
    ExcelRT™ Reverse Tranion Kit
    100 RXN
  •  

     

    Information
     ExcelRT™ Reverse Tranion Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Tranase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Tranase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse tranase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.

    Features
    • Contains all components for reverse tranion 
    • High yield
    • Thermostable, up to 50°C, during first strand synthesis 
    • High processivity, generating cDNA up to 8 kb 
    • Reduced RNase H ribonuclease activity

     

     

    Contents
    Component


    Volume 

    Reverse Tranase
    (200 U/μl)
    100 µl
    RNase Inhibitor (20 U/μl) 100 µl
    5X RT Buffer (DTT) 500 µl
    dNTPs (10 mM each)200 µl
    Oligo (dT) 20 (50 μM)100 µl
    Random Hexamers (100 μM) 100 µl
    DEPC-Treated H2O 2X1 ml


     

    Storage
    -20°C  for 24 months

    Storage Buffer
    Reverse Tranase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol 

     

     

    MSDS / Datasheet 

     

     

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