smobio ▼ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN |
브랜드 | smobio | ||
---|---|---|---|---|
품명 | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN | |||
CAT. NO | TQ1200 | 분류 | PCR Master Mix | |
규격 | 2 tubes(1 ml/tube) | 재고 | STOCK | |
소비자가(₩) | CAS. NO | |||
온도 | -20℃ | 위험물 | ||
수입발주일 | 수시 발주 | |||
주의사항 |
Information
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage (Fig. 1), even at 37°C for weeks. In addition to high sensitivity and signal intensity (Fig. 2), the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results (Fig. 3), as well as a better compatibility with fast PCR program (Fig. 4).
With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
•High Stability
•Fast Hot Start
•High Sensitivity
•Low Background / High Specificity
•Suitable for Fast Program
•Smart Blue Contrast Dye
Storage
Aliquot to avoid multiple freeze-thaw cycles. Protect from light. -20°C for 12 months
Figure 3. High specificity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) by reducing primer-dimer formation.
The post-amplification melt-curve analysis was examined with 5 ng cDNA as template, relatively higher concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents. The single peak of melt curve displays a homogeneous PCR product amplified by TQ1200, while primer dimer signal was shown in qPCR results from brand A.