BRAND

smobio ▼ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 200 RXN

브랜드 smobio
품명 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 200 RXN
CAT. NO TQ1210 분류 PCR Master Mix
규격 2 tubes(1 ml/tube) 재고 STOCK
소비자가(₩) CAS. NO
온도 -20℃ 위험물
수입발주일 수시 발주
주의사항
  • TQ1210
    ExcelTaq 2X Fast Q-PCR Master Mix (SYBR, ROX)
    200 Rxn


  • Deion

    The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage (Fig. 1), even at 37°C for weeks. In addition to high sensitivity and signal intensity (Fig. 2), the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results (Fig. 3), as well as a better compatibility with fast PCR program (Fig. 4). With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

    tq1100













    Features

    •High Stability
    •Fast Hot Start
    •High Sensitivity
    •Low Background / High Specificity
    •Suitable for Fast Program
    •Smart Blue Contrast Dye
    •With ROX reference dye

    Applications
    •Quantitative real-time PCR
    •Quantitative two-step real-time PCR

    Storage
    Aliquot to avoid multiple freeze-thaw cycles.
    Protect from light.
    -20°C for 12 months


    Contents

    Component


    Volume 

    ExcelTaq™ 2X Fast Q-PCR Master Mix  (SYBR, ROX)2 x 1 ml

     

     

    Figure 1. High stability of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX).

    Stability was analyzed via real-time PCR amplification with 2 ng, 20 pg and 0.2 pg cDNA as template, and TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) stored at -20°C or 37°C for 4 weeks as reagents. The overlapped amplification curves of qPCR reactions from different storage conditions display excellent stability of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX). Inconsistent Ct value and decreased fluorescent intensity were observed in qPCR master mix from brand P.


    Figure 2. High sensitivity of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX).

    (A) The sensitivity assay of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was examined with serially diluted cDNA from 25 ng to 1.4 fg as templates. The amplification curves display that TQ1210 is capable to amplify template as little as 1.4 fg, and over a wide range of template usage.

    (B) The sensitivity of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was higher than that of brand P and brand A. The sensitivity assay was examined with 0.2 fg cDNA as template.


    Figure 3. High specificity of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) by reducing primer-dimer formation. .

     

    The post-amplification melt-curve analyzed with relatively higher concentration of primers (400 nM) reveals the specificity of qPCR reagents. The multiple peaks of melt curve display a nonspecific and a target PCR products. The ratio of nonspecific PCR product to target PCR product represents the specificity of qPCR reagents. The ratio analyzed using TQ1210 (0.5 to 1) was lower than the ratio analyzed from brand P (2 to 1) and A (0.8 to 1), showing high specificity of TQ1210.


    Figure 4. TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is compatible with fast program.

     

    The standard curves were examined using fast program with cDNA ranging from 200 fg to 20 ng as template. The efficiency was calculated according to the slope of standard curve. The efficiency of TQ1210 is approximately 100% showing that TQ1210 is more compatible with fast program as compared to brand A (efficiency: 71.9).

     


     

     

    MSDS / Datasheet 

     

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