smobio ▼ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 200 RXN |
브랜드 | smobio | ||
---|---|---|---|---|
품명 | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 200 RXN | |||
CAT. NO | TQ1210 | 분류 | PCR Master Mix | |
규격 | 2 tubes(1 ml/tube) | 재고 | STOCK | |
소비자가(₩) | CAS. NO | |||
온도 | -20℃ | 위험물 | ||
수입발주일 | 수시 발주 | |||
주의사항 |
Deion
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage (Fig. 1), even at 37°C for weeks. In addition to high sensitivity and signal intensity (Fig. 2), the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results (Fig. 3), as well as a better compatibility with fast PCR program (Fig. 4). With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
•High Stability
•Fast Hot Start
•High Sensitivity
•Low Background / High Specificity
•Suitable for Fast Program
•Smart Blue Contrast Dye
•With ROX reference dye
Applications
•Quantitative real-time PCR
•Quantitative two-step real-time PCR
Storage
Aliquot to avoid multiple freeze-thaw cycles.
Protect from light.
-20°C for 12 months
Contents
Component
Volume
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | 2 x 1 ml |
Figure 3. High specificity of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) by reducing primer-dimer formation. .
The post-amplification melt-curve analyzed with relatively higher concentration of primers (400 nM) reveals the specificity of qPCR reagents. The multiple peaks of melt curve display a nonspecific and a target PCR products. The ratio of nonspecific PCR product to target PCR product represents the specificity of qPCR reagents. The ratio analyzed using TQ1210 (0.5 to 1) was lower than the ratio analyzed from brand P (2 to 1) and A (0.8 to 1), showing high specificity of TQ1210.