TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
200 RXN 
 

Information

 The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity (Fig. 1). The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.













Fig. 1. The amplification plot of real-time PCR with cDNA templates ranging from 76 fg to 20 ng in quantity, analyzed by using TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) for qPCR amplification.


Features
• High sensitivity
• High specificity
• With ROX reference dye


Storage
Aliquot to avoid multiple freeze-thaw cycles.
Protected from light.
-20°C for 12 months 

Fig. 2. SMOBIO’s TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) shows better fluorescent signal as compared to a similar product (Brand A). 

MSDS / Datasheet