Information

Echelons Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.

Sample Volume 

400 μL / sample (50 μL per well, 8 wells per sample)

Sample Number 

10 samples (in duplicate) / 96 well assay

Sample Type

Currently this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.

Product Background 

The assay developed by Echelon Biosciences Inc. uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence. 
This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time. Because the assay is not end point based, it has the advantage of being less susceptible to error arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users equipment.

Tech Data Sheet - TDS _K-4100_ rev8x