BRAND

ICT ▼FAM-FLICA® Caspase 3 & 7 Assay Kit

브랜드 ICT
품명 FAM-FLICA® Caspase 3 & 7 Assay Kit
CAT. NO 94 분류 Assay Kits
규격 100 재고 -
소비자가(₩) CAS. NO
온도 4℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항

FAM-FLICA® Caspase-3/7 Assay Kit


Data
Normal (left) and keratoconus (right) corneal fibroblasts were treated with 200 µM H2O2 for 1 hr, washed, and allowed to recover for 1-3 hrs. The culture media was removed and replaced with 1X FAM-FLICA Caspase-3/7 (kit cat.# 93) solution (in culture media) at 300 µl/well for 1 hr. The cell layer was washed 3 times with 1X wash buffer; 300 µl wash buffer was added to keep the cells from drying. Keratoconus corneal fibroblasts treated with H2O2 (right) show a significant increase in caspase-3/7 activity compared to normal cells (left). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, M.D., Ph.D. Dept. of Ophthalmology, UC Irvine.


Specification
• Reagent name: FAM-DEVD-FMK
• Target: Caspase-3/7
• Excitation/Emission: 492 nm / 520 nm
• Method of Analysis: Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
• Types of Samples: Cell culture
• Storage: 2-8°C
• Shipping: Ships overnight (domestic), International Priority Shipping

Kit Contents

Kit 93: 25 Tests
• FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial, #653
• 10X Apoptosis Wash Buffer, 15 mL, #635
• Fixative, 6 mL, #636
• Propidium Iodide, 1 mL, #638
• Hoechst 33342, 1 mL, #639
• Kit Manual

Kit 94: 100 Tests
• FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials, #653
• 10X Apoptosis Wash Buffer, 60 mL, #634
• Fixative, 6 mL, #636
• Propidium Iodide, 1 mL, #638
• Hoechst 33342, 1 mL, #639
• Kit Manual


Documentation

 

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