BRAND

Brand

ENZO ▼Caspase-3 (human), (recombinant) (active)

 브랜드 ENZO
품명 Caspase-3 (human), (recombinant) (active)
CAT. NO ALX-201-059-U100 분류 Protein
규격 1 x 100U 재고 -
소비자가(₩) CAS. NO
온도 -80℃ 위험물
수입발주일 매주 목요일 오후 5시
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Alternative Name:CPP32, Yama, Apopain
 
Source:Produced in E. coli. Contains an N-terminal His-tag.
 
EC:3.4.22.56
 
UniProt ID:P42574
 
Formulation:Lyophilized.
 
Quality Control:Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-pNA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032).
 
Specific Activity:≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
 
Application Notes:Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215).
 
Reconstitution:Reconstitute to 1U/µl with PBS containing 15% glycerol.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.




Manual  / MSDS

 

 

 

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