BRAND

ICT ▼FAM-FLICA® Caspase 8 Assay Kit

브랜드 ICT
품명 FAM-FLICA® Caspase 8 Assay Kit
CAT. NO 99 분류 Assay Kits
규격 25 재고 -
소비자가(₩) CAS. NO
온도 4℃ 위험물
수입발주일 매주 목요일 오후 5시
주의사항

FAM-FLICA® Caspase-8 Assay Kit


Data
ICT’s Caspase-8 FLICA kit was used to detect apoptosis in U937 cells, a histiocytic lymphoma cell line. Cells were treated with a control, 50 ng/mL TNF, or 50 µg/mL cycloheximide (CHX) for 24 hours. Cells were then incubated with FAM-LETD-FMK for 1 hour, washed 4 times, then analyzed on a flow cytometer. FLICA revealed that 41.9% of cells treated with CHX had caspase-8 activity, compared with 19.1% of cells treated with TNF, and only 1.9% of the control cells (Ms. Jennifer Mitchell, Scripps).

Specification
• Reagent name: FAM-LETD-FMK
• Target: Caspase-8
• Excitation/Emission: 492 nm / 520 nm
• Method of Analysis: Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
• Types of Samples: Cell culture
• Storage: 2-8°C
• Shipping: Ships overnight (domestic), International Priority Shipping

Kit Contents

Kit 99: 25 Tests
• FLICA Caspase-8 Reagent (FAM-LETD-FMK), 1 vial, #656
• 10X Apoptosis Wash Buffer, 15 mL, #635
• Fixative, 6 mL, #636
• Propidium Iodide, 1 mL, #638
• Hoechst 33342, 1 mL, #639
• Kit Manual

Kit 910: 100 Tests
• FLICA Caspase-8 Reagent (FAM-LETD-FMK), 4 vials, #656
• 10X Apoptosis Wash Buffer, 60 mL, #635
• Fixative, 6 mL, #636
• Propidium Iodide, 1 mL, #638
• Hoechst 33342, 1 mL, #639
• Kit Manual

 

 

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