BRAND

Brand

LSbio ▼Mouse/Human/Rat GLP1 / Glucagon-Like Peptide 1 ELISA Kit (Competitive EIA) - LS-F412

 브랜드 LSbio
품명 Mouse/Human/Rat GLP1 / Glucagon-Like Peptide 1 ELISA Kit (Competitive EIA) - LS-F412
CAT. NO LS-F412-1 분류
규격 1 plate 재고 -
소비자가(₩) CAS. NO
온도 위험물
수입발주일 매주 목요일 오후 5시
주의사항


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견적문의
Type
 
Competitive EIA (enzyme immunoassay) kit
Target
 
GLP1 / Glucagon-Like Peptide 1
Reactivity
 
Mouse, Human, Rat
Specificity
 
The capture antibody in this kit recognizes human, mouse and rat GLP1 / Glucagon-Like Peptide 1, a cleavage product of GCG / Glucagon. It is specific for aa108-128 (P01275). This will include the active forms 7-36a and 7-37 as well as the inactive cleaved form 9-36a.
Manual
 
Intended Sample Types
 
Cell Culture Supernatants, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Protocol
 
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl anti-GLP-1 antibody to each well. Incubate 1.5 hours at room temperature or overnight at 4°C.
  3. Add 100 µl standard or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
  4. Add 100 µl prepared streptavidin solution. Incubate 45 minutes at room temperature.
  5. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
  6. Add 50 µl Stop Solution to each well. Read at 450 nm immediately
Restrictions
 
For research use only.

 

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