Information
The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.

Additional Format
The SMO-HiFi™ DNA Polymerase is also available in a master mix format. The SMO-HiFi™ 2X PCR Master Mix (Cat. No. TF1100) is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of primers and template. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors

Features
• 5→3 DNA polymerase activity
• 3→5 exonuclease activity (proofreading)
• High reaction rate (up to 1 kb/10 second)
• High fidelity, 70 times higher than Taq DNA polymerase
• Blunt end amplicon
• Thermo-stable-half life is more than 10 hrs at 95°C


Fig. 1. SMO-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 12 kb in length (M: DM5100).

Contents
TF1000 SMO-HiFi™ DNA Polymerase
Component

Volume 

Storage
-20°C for 24 months


TF1100 SMO-HiFi™ 2X PCR Master Mix, 50 Rxn
Component

Volume 

Storage
4°C for 6 months

MSDS / Datasheet