smobio ▼ExcelTaq™ Hot Start II DNA Polymerase (5 U/μl) |
브랜드 | smobio | ||
---|---|---|---|---|
품명 | ExcelTaq™ Hot Start II DNA Polymerase (5 U/μl) | |||
CAT. NO | TP5000 | 분류 | PCR Enzymes | |
규격 | 500 U | 재고 | STOCK | |
소비자가(₩) | CAS. NO | |||
온도 | -20℃ | 위험물 | ||
수입발주일 | 수시 발주 | |||
주의사항 |
Information
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction (Fig. 1). The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C (Fig. 2). The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity (Fig. 3) and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA (Fig. 4) or 1 fg of plasmid DNA (Fig. 5). With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
Features
•Aptamer-based hot start PCR
•Reversible enzyme inactivation
•Omits extra enzyme activation step
•Convenient for room temperature PCR set-up
•High yield and specificity of target amplicons
•Wide range of amplicon length (up to 10 kb)
•High sensitivity (as low as 1 fg of plasmid)
Contents
Component
Volume
Hot Start II DNA Polymerase (5 U/μl) | 100 μl |
10X HS Buffer | 2 x 1 ml |