Information
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction (Fig. 1). The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C (Fig. 2). The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity (Fig. 3) and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA (Fig. 4) or 1 fg of plasmid DNA (Fig. 5). With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Features
•Aptamer-based hot start PCR
•Reversible enzyme inactivation
•Omits extra enzyme activation step
•Convenient for room temperature PCR set-up
•High yield and specificity of target amplicons
•Wide range of amplicon length (up to 10 kb)
•High sensitivity (as low as 1 fg of plasmid)

Contents
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Volume 

MSDS / Datasheet