Information

Echelons Hyaluronidase Activity ELISA is a quantitative immunoassay designed for in vitro measurement of hyaluronidase activity in biological samples.

Background

Hyaluronidases are a group of enzymes that degrade Hyaluronan (HA), a linear polysaccharide comprised of a repeating disaccharide of N-acetylglucosamine and D-glucuronic acid. HA is involved in many biological processes including structural support, cell migration and tissue turnover. Circulating HA levels have also been shown to correlate in several disorders such as liver disease and certain types of cancer. Hyaluronidases have become an important area of study due to their regulatory function in HA metabolism and their role in physiological processes such as fertilization and wound healing. Elevated expression levels of the gene encoding Hyaluronidase 1 have been found in atypical ductal hyperplastic tissues, suggesting the possibility that it may be a viable biomarker for identifying premalignant lesions that can develop into breast cancer. Research has also shown that hyaluronidases are involved in several pathological processes such as bacterial pathogenesis, the spreading of toxins/venoms and cancer progression, making hyaluronidases a potential pharmacological target.

Echelons Hyaluronidase Activity ELISA is a quantitative immunoassay designed for in vitro measurement of hyaluronidase activity in biological samples. Hyaluronidase reactions are performed in a 96-well microtiter plate pre-coated with HA substrate. The activity of the hyaluronidase is determined by comparing HA substrate levels post reaction to a standard curve of pre-coated HA substrate amounts. Echelons Hyaluronidase Activity ELISA has been validated with human sera, plasma and urine samples in addition to purified hyaluronidase from bovine testes. Echelons Hyaluronidase Activity ELISA provides a robust and simple method for researchers to measure hyaluronidase activity in biological samples.

Tech Data Sheet - TDS_K-6000_Rev 1x