Information

The HA ELISA, a competitive 96-well assay, offers a simple, effective method for determining Hyaluronic Acid (HA) levels in human and animal biological fluids or cell supernatants.

Assay Range

50-1600 ng/ml

Sensitivity

25 ng/mL

Sample considerations 

The HA ELISA detects HA molecules that are as small as 6.4 kDA

Product Background

Hyaluronic acid (HA) is a high molecular weight anionic polysaccharide (1,000-10,000 kD) composed of repeating disaccharides and is one of several glycosaminoglycan components of the extracellular matrix of connective tissue. 
Free HA is taken up by the liver where it is degraded and recycled. Many chronic liver diseases, including infection (hepatitis B or C), toxicity (alcohol and drugs), genetic (hemochromatosis), autoimmunity, and malignancy, result in liver inflammation which can progress to liver fibrosis and cirrhosis; causing impairment of liver function and resulting in a rapid increase in circulating HA levels. 
Data indicates a relationship between HA levels, local inflammation and severity of disease. Recent publications have also shown that HA levels in urine are indicative of bladder cancer, that HA levels are directly correlated to liver disease, and suggests enhanced breakdown of HA in the lungs of patients with chronic obstructive pulmonary disease. In addition, serum levels of HA have been found to be elevated in patients with rheumatoid arthritis.

Tech Data Sheet - TDS_K-1200_Rev15