Echelon ▼PTEN Activity ELISA |
브랜드 | Echelon | ||
---|---|---|---|---|
품명 | PTEN Activity ELISA | |||
CAT. NO | K-4700 | 분류 | ELISA kit | |
규격 | 1kit | 재고 | - | |
소비자가(₩) | CAS. NO | |||
온도 | -20℃ 4℃ | 위험물 | ||
수입발주일 | 매주 금요일 오후 4시 | |||
주의사항 | The kit comes in two parts with different storage requirements. Upon receipt store Kit Part 1 at 4 °C and Kit Part 2 at -20 °C. Store prepared reagents as indicated in the protocol |
Information
The PTEN Activity ELISA is designed to quantify
the phosphatase activity of PTEN by detection of the product, PIP2, in a competitive ELISA format, eliminating the need for
radioactivity, organic solvents, and thin layer chromatography. The assay can
be used to measure recombinant or immunoprecipitated PTEN activity.
PTEN (Phosphatase and Tensin Homologd on
Chromosome 10) is a 3’ phosphoinositide phosphatase that converts PI(3,4,5)P3 to PI(4,5)P2, thus opposing
PKB/Akt activation by PI 3-K. PTEN is involved in neuronal stem cell
proliferation and self-renewal, cardiac myocyte hypertrophy and contractility,
and a wide range of developmental processes. PTEN, however, is best known for
its role as a tumor suppressor. Loss of PTEN activity results in accumulation
of PI(3,4,5)P3, abnormal
activation of PKB/Akt, unregulated cell growth, suppression of apoptosis, and
increased tumorigenesis in a number of human tissues. It has also been proposed
that PTEN is a candidate for targeted chemotherapy because certain anti-cancer
agents preferentially destroy tumors with PTEN mutations. In addition to this
direct role in cancer, PTEN has recently been shown to regulate
cancer-associated pathways including VEGF-mediated angiogenesis and others.
The PTEN Activity ELISA directly detects the phosphoinositide
product compared to other assays, which detect free phosphate. This eliminates
many possible sources of error due to the fact that inorganic phosphate is the
product of many phosphatase enzyme activities, and is found in common buffers
and cleaning products. The assay is a competitive ELISA in which the signal is
inversely proportional to the amount of PI(4,5)P2 produced. After the PTEN reactions are complete,
reaction products are added to the PI(4,5)P2-coated microplate
and a PI(4,5)P2 detector
protein is then added for competitive binding. A peroxidase-linked secondary
detector and colorimetric detection is used to detect PI(4,5)P2 detector
binding to the plate. The colorimetric signal is inversely proportional to the
amount of PI(4,5)P2 produced by PTEN.