Information 
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse tranion and PCR amplification of a specific target gene from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides users an alternative to two lengthy steps (first strand generation and amplification) by using a single mixture in a single tube for the one-step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+, enzyme stabilizer, and a blend of recombinant reverse tranase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows users to complete the RT-PCR process using a thermocycler in a single reaction setting and is ideal for target RNA amplification. This product is capable of detecting even trace amounts of target RNA.

Features
• Generates dsDNA directly from RNA samples
• High sensitivity and yields
• High reproducibility, reduced pipetting errors
• High throughput
• Reduced RNase H ribonuclease activity

Contents
Component

Volume 

* RP1100 is optimized for sequence specific primers.
** Adding RNA template last is recommended.

Storage
-20°C for 24 months



Fig.1. ExcelRT™ 2X One-Step RT-PCR Kit can synthesize PCR product with minimal amount of total RNA in reaction solution. The bands in the gel represent the results of GAPDH (230 bp) from one-step RT-PCR reaction. (M: DM2100 marker)

MSDS / Datasheet